Send Out Tests

SECTION ELEVEN
Dahl-Chase Diagnostic Services
SEND-OUT TESTS

Calculi:

For crystallographic analysis of calculi. 
This testing is done at Quest Diagnostics Laboratories for Dahl-Chase routinely on renal stones and ureteral stones.  It may also be ordered on bladder, prostate, salivary gland, and gallbladder calculi.
– Specimen should be received fresh or in saline and sent as dry as possible.

Cytogenetics Testing:

This testing is sent to Affiliated Laboratory, Inc. by Dahl-Chase.

  • Tissues that may be sent include small fragments of villous tissue, skin, or pericardium from a fetus, or as indicated for a specific test
  • The tissue should be placed in RPMI pink tissue medium at the time of collection whenever possible.  Otherwise, the tissue needs to be received fresh, sterile in saline.
  • An ALI genetics requisition should be submitted with the specimen.
  • Formalin must not be used as this will make the specimen unacceptable for cytogenetics.

Eyes:

This testing is to provide special studies on eyes not performed at Dahl-Chase Diagnostic Services.
The specimen is the entire globe or corneal transplant and is sent to the Massachusetts Eye and Ear Infirmary.

  • Specimen must be fixed in 10% buffered formalin for a minimum of 24 hours.
  • A Pathology Information form and Requisition from the Massachusetts Eye and Ear Infirmary needs to be completed by the clinician and it must accompany the specimen to Dahl-Chase.
  • There is an example of these forms following the list of send outs.

Implants:

Bone Implants and Prosthesis are sent to the Thayer School of Engineering for further studies.

  • The implant/prosthesis and an Implant retrieval form completed by the surgeon are sent to Dahl-Chase for transport to Thayer.
  • There is an example of the Implant Retrieval form following this list of send out testing.

Liver Biopsy for Iron or Copper studies:

This testing is done at Mayo Medical Laboratories for Dahl-Chase Diagnostic Services.

  • Need to be sent dry and fresh in a metal free container provided by the grossing room (call941-8252)
  • A minimum of 2 mg of liver tissue is required.  Needle biopsies should be at least 0.5cm long
  • If routine processing is required, a separate piece of tissue should be submitted in formalin.

Muscle Biopsies for Muscular dystrophy and inflammatory muscle disorders:

This testing is done at Fletcher Allen Health Care for Dahl-Chase. 
It is used in the diagnosis of muscular dystrophy (in children) and inflammatory muscle disorders (in adults). 
Muscle tissue is obtained and transferred to Dahl-Chase Diagnostic Services for shipment.

  • Arrangements must be made with Dahl-Chase Diagnostic Services 24 hours in advance of the procedure by calling 941-8252.  Uniship courier must be called in advance to pick up the specimen (848-7546).
  • Muscle biopsy clamps are necessary for these specimens and arrangements must be made in advance to ensure the clamps are available.
  • A Dahl-Chase requisition should be completed thoroughly and submitted with the specimen.  It is imperative that a detailed clinical history including the biopsy site be on the requisition.
  • The turn around time for this test is approximately 2 weeks from the date ordered.

 Specimen requirements:

  • Two biopsies should be taken from the same site with muscle clamps.
  • A third biopsy, if possible, unclamped, should be sent for possible biochemical studies. 
  • The size of the biopsy is important.  Each piece should be about 1x1x0.5cm.
  • If clinically warranted (ex. Dermatomyositis), a piece of skin may also be submitted.
  • Once removed, the biopsies should be placed between gauze dampened with saline.
  • Place the specimens in a bag labeled with the patient’s full name and tissue source. 
  • Place on wet ice in a Styrofoam container and send with Uniship.

Renal biopsies for light microscopy, immunofluorescence, or electron microscopy:

This testing is done at Mayo Clinic Renal Pathology Laboratory for Dahl-Chase. 

  • Need either percutaneous needle biopsies or wedge biopsy.
  • Submit fresh or in saline soaked gauze.
  • Immediate delivery is required.
  • Submit requisition stating what testing is requested along with a completed Renal biopsy data form from Mayo Clinic Renal Pathology Lab available by calling 941-8252.  Please see example of this form following this list of send out testing.
  • The turn around time for this test is approximately one week to ten days from the date ordered.

Skin for immunofluorescence:

This testing is done at Mayo Medical Laboratories Dermatopathology department for Dahl-Chase
It is direct immunofluorescence (IF) studies of skin biopsies to detect IgG, IgM, IgA, C3 and fibrinogen deposition.

  • Need at least 4mm of skin (punch biopsy), taken immediately adjacent to (but not from) the lesion.
  • Submit in Michels medium (order by calling 941-8252) or in saline soaked gauze.
  • Immediate delivery is required.
  • State on requisition that the specimen is for immunofluorescence and include a completed requisition from Mayo Medical Laboratory (available through Dahl-Chase by calling 941-8252) Submit separate piece of skin biopsy from the lesion in formalin if routine pathology is also needed.
  • Questions? Call (207) 941-8252.
  • Turn-around time for this test is approximately 3 days from the date of send-out.

Sural Nerve Biopsy:

This testing is done at Fletcher Allen Health Care for Dahl-Chase. 
The sural nerve biopsy will be fixed for electron microscopy and histology as soon as possible after removal from the patient.

  • Notify Dahl-Chase 24 hours in advance by calling 941-8252.
  • Notify Uniship courier service that this specimen needs immediate attention upon arrival at Dahl-Chase Diagnostic Services. (courier phone 848-7546)
  • The turn around time for this test is approximately 3-4 weeks from the date ordered.

Specimen requirements:

  1. A 3 cm portion of nerve is obtained from the patient and sent to Pathology on a piece of gauze dampened in saline.  Transport on wet ice to Dahl-Chase.
  2. This specimen is fresh and needs immediate attention once it reaches Pathology.

HPV Testing on Tissue blocks

This testing is ordered by Dahl-Chase and sent to Mayo Medical