Immunohistochemistry, Special Stains, In-situ hybridization

Special Stains

Special Stains Alcian Blue/pH 2.5: stains both sulfated and carboxylated acid mucopolysaccharides and sulfated and carboxylated sialomucins (glycoproteins). Alcian Blue/PAS: Acidic mucosubstances are stained with the alcian blue technique and neutral mucosubstances are stained by the PAS reaction. AFB (Ziehl-Neelsen): for the demonstration of acid-fast organisms. Argentaffin Granules, Fontana-Masson: for the demonstration of argentaffin substances such as melanin, argentaffin […]

Panels, Double, and Triple Stains

Panels, Double, and Triple Stains PANELSLung Panel (P40, TTF1 – Napsin A):   Used to differentiate between lung adenocarcinoma and squamous cell carcinoma of the lung.  DOUBLE STAINSPan Keratin – p63:  For evaluation of microinvasion in small breast biopsies Cytokeratin 34β12 (K903) – Racemase (p504s):  For evaluation of small foci of invasive prostate carcinoma P40 – Cytokeratin 5/6: For evaluation

In-Situ Hybridization

In-Situ Hybridization Testing EBV-ISH: is used for the detection of early RNA transcript of Epstein-Barr Virus (EBV) infection. Kappa-ISH: is intended to detect cytoplasmic mRNA for Kappa immunoglobulins light chain in tissue. Combined with Inform Cytoplasmic mRNA Probe for Lambda, the assays assist distinction between polyclonal and monoclonal proliferations of plasma or B cells. Lambda-ISH: is intended to

Immunohistochemistry Testing

Immunohistochemistry Testing AFP: has been immunohistochemically demonstrated in yolk sac carcinomas of gonadal and extragonadal site, in hepatic malignancies and a few other neoplasms. ALK-1: labels normal human ALK protein and the NPM-ALK chimeric protein, and it is a useful tool for the identification of the subgroup of anaplastic large-cell lymphomas (ALCL) that are ALK positive. Arginase-1: