Colorectal Carcinoma Molecular Analysis

NRAS Mutation Detection Clinical Significance:The American Society of Clinical Oncologists (ASCO), ASCP, CAP, AMP, and National Comprehensive Cancer Network (NCCN) recommend routine testing for RAS mutation at diagnosis of stage IV disease and all colorectal carcinomas considered for therapy with cetuximab or panitumumab, either alone or in combination with other anti-cancer agents. https://www.nccn.org/professionals/physician_gls/pdf/colon.pdf Specimen Requirements and Collection:Formalin fixed […]

Microsatellite Instability (MSI) Analysis Clinical Significance:Recently established guidelines from the American Society for Clinical Pathology (ASCP), College of American Pathologists (CAP), Association for Molecular Pathology (AMP) and/or the American Society of Clinical Oncology (ASCP) recommend microsatellite Instability (MSI) testing for all newly diagnosed patients with colon cancer. MSI-H tumors are reflexed to MMR IHC testing and BRAF V600 mutation,

KRAS Mutation Detection Clinical Significance:The American Society of Clinical Oncologists (ASCO), ASCP, CAP, AMP, and National Comprehensive Cancer Network (NCCN) recommend routine testing for RAS mutation at diagnosis of stage IV disease and all colorectal carcinomas considered for therapy with cetuximab or panitumumab, either alone or in combination with other anti-cancer agents. https://www.nccn.org/professionals/physician_gls/pdf/colon.pdf Specimen Requirements and Collection:Formalin fixed

HER2 Gene Amplification Clinical Significance: The American Society of Clinical Oncologists (ASCO), ASCP, CAP, AMP, and National Comprehensive Cancer Network (NCCN) recommend HER2 gene amplification status determination on colorectal cancer patients inappropriate for intensive systemic therapy in a setting of advanced or metastatic disease. Anti-HER2 therapy is only indicated for HER2-amplified tumors that are also RAS and BRAF wild-type. https://www.nccn.org/professionals/physician_gls/pdf/colon.pdf Specimen Requirements and Collection:This

Colorectal Carcinoma Molecular Analysis Clinical Significance:Recently established guidelines from the American Society for Clinical Pathology (ASCP), College of American Pathologists (CAP), Association for Molecular Pathology (AMP) and/or the American Society of Clinical Oncology (ASCP) recommend testing for all resected colorectal carcinomas or metastatic colorectal carcinomas and selected biopsy cases where resection will not be performed

BRAF V600 Mutation Detection Clinical Significance:BRAF V600 mutation analysis is useful in determining patient eligibility for targeted EGFR inhibitor therapy for metastatic colorectal carcinoma and for Lynch syndrome reflex testing (in colorectal carcinomas). https://www.nccn.org/professionals/physician_gls/pdf/colon.pdf Specimen Requirements and Collection:Formalin fixed tissue containing a sufficient amount of tumor (generally at least several mm of tumor tissue submitted in