Unacceptable Specimens for Flow Cytometry Most specimens for flow cytometric analysis should be analyzed within 24 hours to maintain optimal viability of the cells. This may not always be possible and the ordering labs should contact the flow cytometry lab to determine the best course of action to transport the specimen to the lab as […]
Out of state clients use this requisition when sending PNH testingParoxysmal Nocturnal Hemoglobinuria (PNH) Requisition for Out of State Clients Paroxysmal Nocturnal Hemoglobinuria (PNH) Clinical Significance:Paroxysmal nocturnal hemoglobinuria (PNH) is a rare but life threatening and debilitating disease. Clinically, PNH is characterized by chronic intravascular hemolysis, bone marrow failure and life threatening thrombosis. PNH evolves
Leukemia and Lymphoma Evaluations Clinical SignificanceLeukemia and lymphoma populations display characteristic patterns of antigen expression (CD antigens) on the cell surface or in the cytoplasm. Flow cytometric analysis provides increased sensitivity and facilitates identification and proper classification of the hematopoietic neoplasm. This information plays an important role in diagnosis and treatment decisions. Who should be
Immunodeficiency Evaluation Clinical Significance:Immunodeficiency states can be acquired or congenital. Who should be tested?Patients with suspected acquired or congenital immunodeficiency Antibody Panels: Specimens:1-2 ml peripheral blood in EDTA (20°C) Forms:Dahl-Chase Flow Cytometry requisition form along with demographic sheetCBC results obtained from the same tube Transport:Specimens need to be delivered within 24 hours.Flow Cytometry lab should
Fetal Hemoglobin Detection by Flow Cytometry Clinical Significance:Measurement of fetal cells has traditionally been performed based on the Kleihauer-Betke technique. This is a slide-based microscopic counting method of blood smears, which is laborious and suffers from lack of sensitivity (0.5% Fetal cells, approximately 25ml fetal bleed), subjectivity and precision (CV 50-100%). Flow cytometry is capable
DNA Ploidy and Cell Cycle Analysis Clinical SignificanceNuclear DNA content can be measured and analyzed by flow cytometry to determine the DNA ploidy of the sample. Normal cells have a diploid DNA content while many tumors show cells with an abnormal DNA content (aneuploidy). Cell cycle analysis may be used to determine the proliferative activity
